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These symptoms are generally short-lived and tend to fade as the body adapts to elevated growth hormone levels

Leucas zeylanica, which is used in traditional medicine for the treatment of GA, can also improve GA inflammation by reducing the metabolite levels through the inhibition of the AA lipoxygenase pathway (225)

Real-time PCR was carried out using SYBR Green PCR Master Mix (RR091A, Takara, Japan) and the quantitative analysis was performed using LightCycler Roche 480 qPCR instrument

Cells were fixed with 4% PFA for 30 minutes, permeabilized with 0.1% Triton X-100, and blocked with 5% normal goat or donkey serum

Furthermore, it can be also used to characterize symbiotic interactions between the host and its microbiome (166)