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Studies published in 2024 reveal that inositol supplementation helps ameliorate abnormal liver fat metabolism and supports hepatic cellular repair mechanisms, making it a vital ingredient to round out the detoxification process [5] (Zhang et al., 2024)

Then, 200 L of chloroform was added, and the mixture was shaken for 15 s

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Lyse cells by homogenization or sonication in 1-2 mL of cold buffer containing 50 mM MES or phosphate (pH 6-7) and 1 mM EDTA