Prediction of novel therapeutics for emphysema In order to identify compounds that might reverse the gene-expression pattern associated with progression of emphysema, we utilized the CMap [13], a compendium of microarray experiments that measure the effect of therapeutic compounds on gene expression in cancer cell lines
Using HEK-293 adherent cells and K-562 suspension cells treated with varying concentrations of menadione or l-Buthionine Sulfoximine (BSO), two compounds known to affect total glutathione (GSH + GSSG) levels, we assayed the cells in separate assays and in a multiplex assay
Choice of primary antibodies is justified in the Discussion
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