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monocytogenes has perhaps the most complex relationship with GSH that has been described, as it not only imports GSH as a cysteine source with a dedicated GSH transporter 19 , but it senses the host reducing environment and synthesizes its own GSH that post-translationally modifies and activates the master virulence transcription factor PrfA 16,17,18

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This so-called within-batch effect, decreases the repeatability and reproducibility as well as the discriminant power to detect relevant biological responses, and difficults data interpretation and reuse