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Mouse and rat data were merged directly

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Cells were loaded with MitoTracker Red dye (Excitation/Emission: 579/599 nm) at 100 nM final concentration (37 C for 15 min) mounted in Live Cell Imaging Solution (Thermo Fisher), and viewed under a laser confocal microscope (LSM 510

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*Strength of evidence (*weak, **moderate, ***strong) supporting neutrophil serine protease dysfunction as a key etiologic factor