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The length of time a B12 shot lasts depends on why you need it

doi: 10.1111/j.1468-1293.2010.00906.x

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The first day is the most important as the Botox starts to take effect

But only a maximum of 5mcg of B12 enters the bile every day (according to "Advanced Haematology" 7th edition textbook, page 60), so it seems that only an additional 5mcg would be lost daily if none was recovered

Body weight, first thing in the morning after using the bathroom, same scale every time