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Both deamination of Gln and degradation of GSH by Helicobacter GGT generate glutamate 15 , 16 , 20 , which can be taken up in the bacterial cell by a Na + -dependent transporter 20 , 29

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Cycle threshold (Ct) values of target genes were calibrated to the Ct for the corresponding genes obtained for samples from the control pCYC1-GAP1 strain and normalised to the geometric mean of the reference genes for relative quantification using the comparative Ct method [36]