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Mobile phase A was 95% water with 5% acetonitrile (containing 0.1% formic acid), and mobile phase B was 7.5% acetonitrile, 47.5% isopropanol, and 5% water (also with 0.1% formic acid)

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Allow the lyophilized vial to equilibrate to ambient room temperature before removing the seal to prevent condensation within the vial

Note the tears at the insertion point

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