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It ensures tissues get enough oxygen

The tissues were lysed in sonication buffer (20 mM HEPES pH7.5

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All animal studies were conducted in accordance with the International Guiding Principles for Biomedical Research Involving Animals and were approved by the Animal Care and Use Committees of Chiba University and the National Institute for Physiological Sciences in Japan

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Hematocrit and reticulocyte counts should be repeated daily from the fifth to seventh days of therapy, and then frequently until the hematocrit is normal