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By binding directly to actin monomers, TB4 prevents the premature polymerization of globular actin (G-actin) into filamentous actin (F-actin)

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In addition, several previously identified OA genetic loci appear to confer risk for the development of OA across multiple joints: these include loci for TGF, RUNX2, COL27A1, ASTN2, IL11, and GDF5 [25]

The scalability of this purification technique allows for the production of microgram, milligram, or even gram quantities of protein, catering to various experimental needs and research scales