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Supplementary Validation Method: LCMS/MS For high-sensitivity detection requirements, liquid chromatographytandem mass spectrometry is used for supplementary validation, especially suitable for trace component analysis and metabolic studies

These pathway interactions are typically evaluated using gene expression assays, protein phosphorylation measurements, and molecular signaling analyses in controlled experimental systems

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This paradigm shift in peptide engineering combines data-driven insights with traditional rational design principles, substantially expanding the landscape of feasible peptide therapeutics