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The administration of high doses of lipoic acid by injection to rats decreased the activity of two biotin-dependent enzymes by about 30%-35% (122), but it is not known whether oral or intravenous lipoic acid supplementation substantially increases the requirement for biotin in humans (123)

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Further research has shown that Erastin and similar ferroptosis inducers induce cell death by reducing the synthesis of GSH, leading to the inactivation of GPX4