To isolate cardiac myocyte from C57BL/6 mice, cardiac tissue samples were digested using collagenase and maintained in media containing 10% FBS and penicillin/streptomycin, with cardiac myocytes being purified via differential applanation
J Clin Endocrinol Metab (1993) 77:16228.10.1210/jcem.77.6.8117355 8 SimpsonERMahendrooMSMeansGDKilgoreMWHinshelwoodMMGraham-LorenceSet alAromatase cytochrome P450, the enzyme responsible for estrogen biosynthesis
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Experiments were performed at room temperature with glass pipettes (4-5M resistance) filled with the intracellular solution comprising 140 mM CsMeSO 3 , 4 mM NaCl, 1 mM MgCl 2 , 1 mM EGTA, 5 mM HEPES, 2 mM MgATP, and 0.6 mM NaGTP, pH = 7.4 (CsOH)
Th Du Mt, Bnh Dng (Cnh m cay Sasin)
