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After the mixture cooled down, the absorbance value at 500 nm was measured using a microplate reader

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Using tissue from donors, the team observed that cells in the nucleus pulposus, a jelly-like structure at the center of intervertebral discs, had a lower amount of this peptide receptor when degeneration increased

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This account does not serve as a substitute for professional medical advice/help.

Acknowledgements Not applicable Funding This work was supported by grants: Zhejiang basic public welfare research program (Grant Numbers LGD21H250001 and LGD22H250001), Scientific Research Foundation of Zhejiang University City College (No.X-202102)