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Cryo-EM grid preparation and data collection For the cryo-EM grids preparation, 3 l of purified ghrelin-bound complex at 13 mg/ml and GHRP-6-bound ghrelin receptor complex at 8 mg/ml were applied individually onto a glow-discharged holey carbon grid (Quantifoil, Au300 R1.2/1.3) in a Vitrobot chamber (FEI Vitrobot Mark IV)

Peng et al., 2022)

10.1096/fasebj.6.11.1644261 11 BagheriG

This can include drug metabolites, heavy metals, pesticides/herbicides, xenoestrogens like BPA, alcohol, and other toxic chemicals