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Hippocampal neurons were maintained in Neurobasal A media from Invitrogen (Carlsbad, CA) supplemented with B27 from Invitrogen, 0.5 raM L-glutamine, and 5mM cytosine-D-arabinofuranoside from Sigma added at 2 days in vitro

Crude protein mixture was than purified through 5 consecutive chromatography steps with different type of chromatography

Consistent Injection Timing Maintaining the same injection day each week helps keep hormone levels stable

Semaglutide shelf life in the fridge is comparable to tirzepatide when stored properly

The Semax dosage guide provides more detailed information on these protocols