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Samples (30 g protein) were resolved by 420% SDS-PAGE (Biofuraw Precast Gel, Tanon Science, Shanghai, China), transferred to polyvinylidene difluoride membranes, and incubated with one of the following antibodies: LC3 (88588s, Cell Signaling Technology, 1:1000), GPX4 (ab125066, Abcam, 1:1000), p16 (1003-1-AP, Proteintech, 1:1000)
For the luciferase assays, 5 L of the supernatant was diluted with 95 L PBS (pH 7.4) in white flat-opaque-bottomed 96-well plates (Thermo Fisher Scientific)
However, other studies have implied that multiple IgG molecules can bind to the same pathogen and that the antibody array on the surface of pathogen allows for multivalent interactions with the mucin network, creating an avidity effect sufficient to effectively trap pathogens 85
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minimal systemic safety data available Learn More Which Peptide for Which Application
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