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Bergenin impedes the generation of extracellular matrix in glomerular mesangial cells and ameliorates diabetic nephropathy in mice by inhibiting oxidative stress via the mTOR/-TrcP/Nrf2 pathway

After culturing, cells were washed three times with PBS, treated with NIB or NIE (200 M) for 5 min at room temperature and imaged using a fluorescence microscope

The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript

J Clin Endocr Metab 63:412-417, 1986

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It would not be feasible to remove these from the diet altogether, such as glycerol 274 , amino acids that lead to acetate production through proteolytic fermentation by gut bacteria 275 , or by the degradation of mucins and other glycoproteins that are secreted by the gut lining 276