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Partial least squares discriminant analysis (PLS-DA) and enrichment analysis PLS-DA and Enrichment Analysis were done with MetaboAnalyst 6.0 (accessed June 2025) 36

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62,63 However, one underlying mechanism is the phosphorylation of Rho A, which inhibits Rho kinase, thereby activating myosin light chain phosphatase, which in turn leads to smooth muscle cell relaxation

Some researchers also include supportive supplements alongside peptide protocols: quercetin as a natural mast cell stabilizer, vitamin D for immune regulation, and omega-3 fatty acids for anti-inflammatory support