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Practical limits depend on sterile techniquemost research protocols target fewer than 30 punctures per vial

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A maximum of 4 burr holes, 2 in each hemisphere, were used to inject pLKO_EGFP_Lox-STOP-Lox_ Gsto2 -shRNA or scrambled shRNA viral vectors by following specific stereotaxic coordinates

10.1021/bi051823 66 PattenC