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The reactions were incubated for one cycle at 42C for 2 minutes and then for another 50 minutes at 42C, after which they were heated for 15 minutes at 70C and chilled at 4C

The peptide also modulates inflammation by shifting macrophage polarization from the pro-inflammatory M1 phenotype to the reparative M2 phenotype , reducing levels of TNF-, IL-6, and IFN- PMID: 40789979

Copper itself is essential for enzymatic processes that protect the skin from oxidative stress