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High-throughput sequencing targeting the V3-V4 hypervariable region of the bacterial 16S rRNA gene was conducted by Shanghai Majorbio Bio-Pharm Technology Co., Ltd

The samples were protected from sunlight and kept in the glove box under N 2 atmosphere (25 2 C) for 12 hours, until the reactions in all the samples had reached equilibrium

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Mice with inflammatory bowel disease healed within days of orally ingesting the peptide

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