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The rate of GSH formation was monitored as the net rate of fluorescence increase of GSH-monochlorobimane adduct catalysed by GST over time after subtracting the BSO-inhibitable fluorescence signal

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McGrail DJ, Garnett J, Yin J, Dai H, Shih DJH, Lam TNA, Li Y, Sun C, Li Y, Schmandt R, Wu JY, Hu L, Liang Y, Peng G, Jonasch E, Menter D, Yates MS, Kopetz S , Lu KH, Broaddus R, Mills GB, Sahni N, Lin SY

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This index did not confirm the negative impact of an 8 days of water-only fasting on kidney functions, because this ratio did not change and did not exceed physiological norms 58