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Snap frozen rat and mouse heart tissues were homogenised using Percellys tubes (approximately 10 mg tissue/300 L 50 mM HEPES (pH 7.4) buffer containing 10 mM IAM-TPP two times for 30 s)

doi: 10.1162/jocn_a_00003, 54

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Puerarin: a review of pharmacological effects

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There is only the case of the nuclear transcription factor c-Jun that, due to an unusual redox potential, could react with GSSG at relatively high ratio of GSH/GSSG = 13 (Klatt et al., 1999)