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Phagocytosis assay THP-1 cells were differentiated into macrophages by 100 nM PMA for 2 days at 37C and incubated with media infused with 1% e-cigarette aerosol solution (PG:VG, 60:40) with and without nicotine and Smoothol (NicQuid) in presence of fluorescein-labeled rabbit IgG-coated latex beads (Cayman Chemical, Ann Arbor, MI) for an additional 3 h following manufacturers instructions

Young adults with family incomes of less than 200% of the federal poverty level also were more likely to use e-cigarettes than those with greater family income