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Glutathione affinity purification, like many other types of affinity purifications, involves three key steps: 1) binding the target protein to the beads, 2) washing away contaminating proteins, and 3) eluting your protein of interest (Figure 3)

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[DOI] [PubMed] [Google Scholar] 19.Tong J., Fitzmaurice P.S., Moszczynska A., Rathitharan G., Ang L.C., Meyer J.H., Mizrahi R., Boileau I., Furukawa Y., McCluskey T., et al