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GLS, the rate-limiting enzyme in glutamine catabolism, exhibits markedly higher expression in AR-insensitive cells, particularly DU-145, contributing to their increased glutamine dependency

It is a chain of amino acids that helps promote healing and recovery, particularly in muscles and connective tissues

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For the FOXO4-DRI treatment group, 50 M FOXO4-DRI (MCE, HY-P4157) was added to the glucose-free DMEM (Gibco, Catalog #11966-025) medium, followed by 3 h of oxygen-glucose deprivation (OGD) treatment

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