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The selected dose of 120 mg/kg body weight is a well-established one for inducing significant and consistent testicular oxidative stress and spermatotoxicity in rodent models, as extensively documented in the literature 39
CRISPR-Cas9-mediated genome editing has successfully generated Brassica varieties with significantly increased glucoraphanin accumulation by modulating key genes in the pathway, including MYB28, AOP2, and BCAT4 (96, 107)
