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Prior to any procedures, animals are anesthetized using a combination of xylazine (10 mg/kg), ketamine (40 mg/kg) and acepromazine (0.75 mg/kg) for up to one hour via the intramuscular route

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Psoriatic skin disease was also improved with adalimumab compared with placebo

Monitoring the effluent at 220 nm, chromatography (flow rate: 18.0 mL/min) was performed starting with a MeOH/H 2 O mixture (85/15, v/v) for 1 min, increasing the MeOH content to 100% within 10 min, holding for 10 min, followed by a decrease of the MeOH content to 85% within 1 min and re-equilibration for 2 min at 85% MeOH