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Normal equilibrium is therefore lost

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The BBB is vital for maintaining brain homeostasis by regulating the exchange of compounds between the blood and the brain parenchyma (Figure 1

Human biological samples and associated data were provided by the Westdeutsche Biobank Essen (WBE, University Hospital Essen, University of Duisburg-Essen, Essen, Germany

Cells were extensively washed with PBS, fixed in 4% PFA for 15 min, permeabilized and blocked with 0.01% saponin/10% normal goat/PBS serum and stained with the primary antibodies in the same blocking solution in a humid chamber

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